10月24日 下午15：30—17：30
新葡的京集团35222vipD912-D914
报告：Use of Genomic Strategies to Determine Mechanisms of Toxicity of oil in Larval Fish
摘要：PAHs elicit cardiotoxicity through activation of the aryl hydrocarbon receptor (AhR) and impaired calcium signaling, but additional pathways of toxicity have been observed including downregulation of genes that regulate potassium and calcium channels in embryonic and larval stages of development. While functional inhibition the channels has been observed following exposure to oil and non-AhR activating PAHs, mechanisms associated with downregulation has not been elucidated. MicroRNAs (miRNA) play key roles in a number of diverse biological processes including heart development in vertebrates. To test the hypothesis whether miRNA changes may regulate ion channel genes, embryos and larvae of mahi-mahi (Coryphaena hippurus) were treated with High Energy Water Accommodated Fractions (HEWAF) made from source and weathered DHW oil. miRNAs and mRNA were sequenced from the same group of pooled animals and expression compared using advanced bioinformatics with subsequent target organ predictions based on their interactions. Gene ontology (GO) analysis on the target mRNAs was consistent with pathway analysis of miRNAs, predicting disruption of cardiovascular system development after oil exposure and showed that specific miRNA–mRNA interactions may contribute to these effects. Oil caused an overexpression of miR-133a, miR-34, and miR-15b. Enhanced expression of miR-133a correlated to the decrease in the expression of KCNH2 mRNA, which controls the potassium ion transporter that has been observed to be reduced in the cardiac phenotype in multiple fish species following oil treatment. In addition miR-34 and 15b, were also upregulated and informatic analyses with mRNAs were consistent impairment of eye development. Analyses of dose response treatments of oil at early hatch larval stages (48 hpf) also predicted cardiac impairment with additional characterization of novel microRNAs. These results indicate that genomic tools can be used to identify and predict novel mechanisms of toxicity of environmental contaminants in biota.
This research was made possible by a grant from The Gulf of Mexico Research Initiative. Grant No: SA-1520; Name: Relationship of Effects of Cardiac Outcomes in fish for Validation of Ecological Risk (RECOVER).
简介：
Professor DANIEL SCHLENK
Environmental Toxicology
Department of Environmental Sciences
University of California, Riverside
EDUCATION
Ph.D. Biochemical Toxicology Oregon State University June 1989
B.S. Toxicology; Northeast Louisiana University(University of Louisiana Monroe) May 1984
Postdoctoral Fellow: Duke University 1989-1991
EMPLOYMENT
2000-present; Professor, Environmental Toxicology, Department of Environmental Sciences, University of California, Riverside
1999-2000 Program Coordinator of Environmental Toxicology Program, Environmental and Community Health Research Program, School of Pharmacy, University of Mississippi
1998-2000 Associate Professor of Pharmacology and Toxicology, University of Mississippi (University, MS).
1998-2000 Coordinator for the Graduate Program in Pharmacology, University of Mississippi (University, MS)
1995-1998 Assistant Professor of Pharmacology and Toxicology University of Mississippi (University, MS).
1991-1995 Assistant Professor Toxicology, University of Arkansas for Medical Sciences (Little Rock, AR).
1989-1991 Postdoctoral Fellow, Duke University Marine Laboratory, Integrated Toxicology Program,(Beaufort, N.C.).
1986-1989 Predoctoral Fellow, Oregon State University, Toxicology Program (Corvallis, OR).
1985-1986 Research Assistant, Oregon State University, College of Pharmacy (Corvallis, OR).
1984-1985 Research Assistant, Oregon State University, Department of Environmental Engineering (Corvallis, OR).
ACADEMIC HONORS
NIEHS Postdoctoral Fellow, Duke University 1989-1991
NIEHS Predoctoral Fellow, Oregon State University 1986-1989
Visiting Scholar Department of Biochemistry; Chinese University of Hong Kong 1995;1998; 1999
Ray Lankester Investigatorship -Marine Biological Association of the United Kingdom 1998
Visiting Scholar of the Instituto Del Mare, Venice Italy 1999
University of Mississippi; School of Pharmacy, Faculty Research Award, 1999-2000.
Member of Eleventh International Pollution Responses in Marine Organisms Symposium Scientific Advisory Committee, Plymouth, United Kingdom 2001; Florianopolis, Brazil 2007; Bordeaux, France 2009; Faro, Portugal 2013.
George E. Brown, Jr. Award (UC-MEXUS) Co-PI with J. Garcia-Hernandez 2001
Visiting Scholar CSIRO Laboratory Lucas Heights, Australia 2003
Invited participant in the Joint SETAC-SOT Pellston Workshop on Emerging Molecular and Computational Approaches for Cross-Species Extrapolations. Portland, OR July 18-22, 2004.
Fellow American Association for the Advancement of Science 2010
Distinguished Fellow of the State Key Laboratory for Marine Environmental Science in Xiamen University of China 2011
Visiting Professor Fellowship of the National Counsel of Technological and Scientific Development at the University of Sao Jose Rio Preto, Brazil 2014-2015.
Outstanding Foreign Scientist Invitation Program; College of Science Sungkyunkwan University, Korea 2016
Fellow Society of Environmental Toxicology and Chemistry 2017
EDITORIAL RESPONSIBILITIES
Associate Editor Environmental Science and Technology (2016-present)
Associate Editor Environmental Science and Technology Letters (2016-present)
Co-Editor in Chief- Aquatic Toxicology (2005-2011)
Editorial Board: Toxicological Sciences (2000-present), Marine Environmental Research (2000-present), Aquatic Toxicology (2001-present), Environmental Toxicology and Chemistry (2003-2005).
